Association of the P561T and C422F polymorphisms of the growth hormone receptor gene with facial dimensions.

Authors

  • Kazem Dalaie Department of Orthodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Mohammad Behnaz Department of Orthodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Samin Banihashem Private pratice, Zahedan, Iran.
  • Saeed Reza Motamedian Department of Orthodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Vahid Reza Yassaee Genomic Research Center, Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Feyzollah Hashemi-Gorji Genomic Research Center, Depart-ment of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Arash Khojasteh Department of Oral and Maxillofacial Surgery, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

DOI:

https://doi.org/10.17126/%25x

Abstract

Background: Growth hormone plays a significant role in determining craniofacial morphology. Mutations of its receptor gene might be associated with mandibular prognathism (MP). Purpose: The aim of the current study was to evaluate growth hormone receptor (GHR) gene polymorphisms in relation to facial dimensions. Material and Method: The study enrolled 65 participants with class III profile in MP group and 60 orthognathic control participants. Genomic DNA was extracted from a blood sample from the patients and the P561T and C422F polymorphisms of GHR gene were screened by PCR-RFLP method followed by Sanger sequencing of randomly selected samples to validate the genotyping results. Chi square was used to compare distribution of polymorphism in MP and control groups (p<0.05). Results: Heterozygous P561T mutation was found in 10.77% and 8.33% of MP and control groups, respectively (p=0.644) while none of the subjects had the C422F mutation. Sanger sequencing confirmed the genotyping results from the PCR-RFLP method. P561T polymorphism was significantly associated with ramus and lower facial height in MP patients and with ramus height in orthognathic patients (p<0.05). Conclusion: The results indicate that the P561T polymorphism of the GHR gene is associated with the vertical dimension of the mandible in an Iranian population.

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Published

2019-12-31

How to Cite

1.
Dalaie K, Behnaz M, Banihashem S, Motamedian SR, Yassaee VR, Hashemi-Gorji F, Khojasteh A. Association of the P561T and C422F polymorphisms of the growth hormone receptor gene with facial dimensions. J Oral Res [Internet]. 2019Dec.31 [cited 2024Jul.22];8(6):499-504. Available from: https://revistas.udec.cl/index.php/journal_of_oral_research/article/view/1937

Issue

Vol. 8 No. 6: November - December

Section

Articles

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