COMBINATION OF ISOLATES OF Pochonia chlamydosporia FOR THE CONTROL OF Meloidogyne javanica IN TOMATO

Abstract

Three different isolates of Pochonia chlamydosporia (Pc-3, Pc-10 and Pc-28) were evaluated in a greenhouse experiment when applied individually and in combinations. A control treatment with no application of isolates of P. chlamydosporia var. chlamydosporia was included, while neither isolates nor nematodes were used in another untreated treatment. One-liter pots were filled with sterilized soil, and each pot was infested with 3 g of rice substrate colonized with the respective isolate plus 3,000 eggs of Meloidogyne javanica. One ‘Santa Clara’ tomato seedling was transplanted to each pot after fifteen days. None of the isolates (applied individually or in combinations) was able to reduce the number of galls when compared to the control treatment. However, the number of eggs of M. javanica decreased for isolates Pc-10 and mixtures Pc-10 + Pc-3, and Pc-10 + Pc-28 + Pc-3. The association of isolates of P. chlamydosporia var. chlamydosporia did not increase the control of M. javanica when compared to the application of Pc-10 individually.