ZINC BIOFORTIFICATION IN LENTIL (Lens culinaris Medik.) SPROUTS: EFFECTS ON PRODUCTIVE AND BIOCHEMICAL PARAMETERS

Abstract

Zinc is an essential micronutrient involved in key biological functions, including enzyme activation and protein synthesis. Its deficiency can lead to malnutrition, impair immune function, and reduce growth rates in children. Therefore, agricultural production should adopt sustainable practices that ensure an adequate zinc supply within food systems. In this context, zinc biofortification of lentil (Lens culinaris Medik.) sprouts emerges as a viable strategy to improve human nutrition without apparent adverse effects. This study evaluated the effect of five zinc sulfate concentrations (0, 25, 50, 100 and 200 µM L^-1) on germination, early growth, and biochemical composition of lentil sprouts. A completely randomized design with four replicates was implemented, with experimental units randomly assigned, each containing 100 seeds placed in glass jars under controlled conditions for nine days. The 50 µM L^-1 treatment promoted sprout growth, improved zinc bioavailability, and reduced phytic acid content, although it did not achieve the highest germination rate. In contrast, the 100 and 200 µM L^-1 treatments increased zinc accumulation but reduced indoleacetic acid (IAA) content, negatively affecting sprout growth. These findings suggest that zinc biofortification in lentil sprouts can improve both nutritional quality and growth performance, representing a feasible and sustainable alternative for enhancing human nutrition.