Advantages of new generation sequencing by synthesis for the study of bacterial communities in root canal therapy.
DOI:
https://doi.org/10.17126/%25xAbstract
Today new generation sequencing (NGS) technologies allow a more accurate analysis of the bacterial composition of a microbiota. Additionally, they have contributed to the understanding of the role that prokaryotes play in endodontic pathogenesis. The sequencing of the hypervariable regions V3 and V4, a segment of the 16S rRNA gene, to evaluate diversity together with sequencing by synthesis (e.g. Illumina MiSeq) has made it possible to elucidate how clinical patterns influence the structure of the bacterial community.
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