Prevalence of HPV and EBV infection and their relationship with the p53 and PCNA expression in oral carcinoma patients

Autores/as

  • Dayahindara Veitía Laboratorio de Genética Molecular, Instituto de Oncología y Hematología- MPPS. Caracas, Venezuela
  • Juan Liuzzi Laboratorio de Genética Molecular, Instituto de Oncología y Hematología- MPPS. Caracas, Venezuela
  • Maira Ávila Servicio de cabeza y cuello del Hospital Oncológico ‘Padre Machado’-IVSS, Caracas, Venezuela
  • Zoraya De Guglielmo Servicio de cabeza y cuello del Hospital Oncológico ‘Padre Machado’-IVSS, Caracas, Venezuela
  • María Correnti Instituto de Investigaciones Odontológicas Raúl Vincentelli, Facultad de Odontología de la UCV, Caracas, Venezuela

DOI:

https://doi.org/10.17126/%25x

Palabras clave:

Carcinoma, VPH, VEB, p53, PCNA

Resumen

Introduction: Infection caused by potentially oncogenic viruses, such as HPV and EBV, favors the role of certain oncoproteins that can induce dysplasias and malignant lesions. Objective: To evaluate the prevalence of HPV and EBV and their relation with the expression of p53 and PCNA in patients with oral carcinoma. Methodology: Twenty-seven oral squamous cell carcinomas (OSCC) were evaluated; DNA extraction was conducted using the QIAamp DNA mini kit; viral detection was obtained using the INNO-LiPA kit for HPV, and nested PCR was used for EBV. The evaluation of molecular markers was performed through immunohistochemical staining. Results: The mean age of the patients was 60.55±13.94 years, and 52% of these were female. Of the patients, 59% were tobacco users and 63% were alcohol consumers. HPV was detected in 70% of the patients with the predominance of genotype 16 (60%). As for EBV infection, it was observed in 59% of cases. p53 and PCNA immunopositivity corresponded to 44% and 59%, respectively. The tongue was the anatomical location with highest positivity for both viruses as well as for the expression of molecular markers. The 48% of the cases presented infection by both viruses. 

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Publicado

2019-07-03

Cómo citar

1.
Veitía D, Liuzzi J, Ávila M, De Guglielmo Z, Correnti M. Prevalence of HPV and EBV infection and their relationship with the p53 and PCNA expression in oral carcinoma patients. J Oral Res [Internet]. 3 de julio de 2019 [citado 25 de junio de 2024];6(4):86-91. Disponible en: https://revistas.udec.cl/index.php/journal_of_oral_research/article/view/894

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