ANTIBIOTIC RESISTANCE OF Salmonella spp. ISOLATED FROM POULTRY MEAT AND THE ANTIMICROBIAL EFFECT OF PLANTS EXTRACTS

Autores/as

  • Franco Guzmán Departamento de Producción Animal, Facultad de Agronomía, Universidad de Concepción, Chillán, Chile
  • Valeria Velasco Departamento de Producción Animal, Facultad de Agronomía, Universidad de Concepción, Chillán, Chile
  • Ana María Bonilla Departamento de Producción Animal, Facultad de Agronomía, Universidad de Concepción, Chillán, Chile
  • Pamela Williams Departamento de Producción Animal, Facultad de Agronomía, Universidad de Concepción, Chillán, Chile
  • Felipe Medina Departamento de Producción Animal, Facultad de Agronomía, Universidad de Concepción, Chillán, Chile
  • Macarena Gerding Departamento de Producción Vegetal, Facultad de Agronomía, Universidad de Concepción, Chillán, Chile
  • Christian Folch Departamento de Agroindustrias, Facultad de Ingeniería Agrícola, Universidad de Concepción, Chillán, Chile
  • Pía Oyarzúa Departamento de Producción Vegetal, Facultad de Agronomía, Universidad de Concepción, Chillán, Chile

DOI:

https://doi.org/10.29393/CHJAAS41-5ROVC80005

Palabras clave:

Antimicrobial resistance (AMR), poultry chain, essential oils, plant extracts, by-products

Resumen

Salmonella spp. is one of the major pathogens causing foodborne disease outbreaks worldwide. It is a priority public health concern due to antimicrobial resistant strains. Poultry is considered to be a major source of transmission of Salmonella spp. The objective of this study was to determine the prevalence and susceptibility of Salmonella spp. strains isolated from poultry to antibiotics and to oregano essential oil (Origanum vulgare L.), chestnut shell extract (Castanea sativa) and grape pomace extract (Vitis vinifera). Samples were collected from chickens, hens and broilers (cloacal swabs) (n=120), poultry meat (n=98) and eggs (n=138). Salmonella spp. were isolated by selective enrichment and culture method and confirmed by polymerase chain reaction (PCR) (identification of hilA gen). ERIC fingerprinting PCR was used to select strains according to their genetic diversity. Susceptibility of selected strains to antibiotics and plant extracts was determined by disk diffusion method and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by dilution method. The total prevalence of Salmonella spp. was 6.53% (23/356) and only meat samples were positive, with a higher prevalence in packaged meat (P ? 0.05). All selected strains were multi-drug resistant, i.e. resistant to three or more classes of antibiotics. Oregano essential oil and chestnut shell extract had antibacterial activity against resistant Salmonella spp. isolates. However, grape pomace extract showed no inhibitory activity. Therefore, the use of oregano essential oil and chestnut shell extract at some stages of the poultry production chain should be evaluated in future studies to have solutions for the industry.

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Publicado

2025-04-30

Cómo citar

Guzmán, F. ., Velasco, V., Bonilla, A. M. ., Williams, P. ., Medina, F. ., Gerding, M. ., Folch, C. ., & Oyarzúa, P. . (2025). ANTIBIOTIC RESISTANCE OF Salmonella spp. ISOLATED FROM POULTRY MEAT AND THE ANTIMICROBIAL EFFECT OF PLANTS EXTRACTS. Chilean Journal of Agricultural & Animal Sciences, 41(1), 33-43. https://doi.org/10.29393/CHJAAS41-5ROVC80005

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Artículos de investigación