Antifungal activity of Crotalaria longirostrata Hook. & Arn. extracts  against phytopathogen fungi from maize

Rosa Isela Cruz-Rodríguez; Rocío Meza-Gordillo; Martha Alicia Rodríguez-Mendiola; Carlos Arias-Castro; Norma Alejandra Mancilla-Margalli; Martín Eduardo Ávila-Miranda; José Miguel Culebro-Ricaldi; Federico Antonio Gutierrez-Miceli; Víctor Manuel Ruiz-Valdiviezo; Teresa del Rosario Ayora-Talavera

Authors

Rosa Isela Cruz-Rodríguez Laboratory of Biotechnology, Instituto Tecnológico de Tuxtla Gutiérrez.
Rocío Meza-Gordillo Laboratory of Biotechnology, Instituto Tecnológico de Tuxtla Gutiérrez.
Martha Alicia Rodríguez-Mendiola Instituto Tecnológico de Tlajomulco Jalisco.
Carlos Arias-Castro Instituto Tecnológico de Tlajomulco Jalisco.
Norma Alejandra Mancilla-Margalli Instituto Tecnológico de Tlajomulco Jalisco.
Martín Eduardo Ávila-Miranda Instituto Tecnológico de Tlajomulco Jalisco.
José Miguel Culebro-Ricaldi Laboratory of Biotechnology, Instituto Tecnológico de Tuxtla Gutiérrez.
Federico Antonio Gutierrez-Miceli Laboratory of Biotechnology, Instituto Tecnológico de Tuxtla Gutiérrez.
Víctor Manuel Ruiz-Valdiviezo Laboratory of Biotechnology, Instituto Tecnológico de Tuxtla Gutiérrez.
Teresa del Rosario Ayora-Talavera Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Unidad Sureste, Parque Científico y Tecnológico del Estado de Yucatán.

Keywords:

Fungicidal properties, extracts, Fusarium verticillioides, Fusarium sp., Aspergillus flavus

Abstract

In the present study, the hexane, acetone and methanol extracts of stem, branches and roots from Crotalaria longirostrata were evaluated for their antifungal activity against Fusarium sp., Fusarium verticillioides and Aspergillus flavus. The variables analyzed were inhibition halos, mycelial growth inhibition, sporulation and minimum inhibitory concentration. Phytochemical analysis revealed the presence of saponins, coumarins, anthrones, anthraquinones, flavonoids and alkaloids on different organic extracts. The hexane extract showed zones of inhibition between 6.3 and 10.5 mm. The mycelia growth and sporulation of A. flavus were reduced to 90% with methanol extract. The minimum inhibitory concentration values obtained with hexane extracts were 6.75 mg mL-1 and with methanol extracts were 50 and 25 mg mL-1 for Fusarium strains. This is the first study reporting of phytochemical composition and biological activity of C. longirostrata that could be used as a natural alternative to control in vitro of certain important pathogenic fungi.