Baybean (Canavalia rosea (Sw.) DC.); organogenesis, morphological and anatomical studies

Abstract

Morphological and anatomical studies comprised leaf venation, histological analysis and epidermal peeling were carried out on Canavalia rosea (Sw.) DC.). Anatomical studies of the leaf and root determined the presence of cuticle and oil glands, arrangement of cells and the structure of vascular system. The dorsiventral leaf with pinnate venation and amphistomatic leaf with paracytic type stomata were embedded below the epidermis. Trichomes and oil glands were also observed on both adaxial and abaxial surfaces of leaf. Longitudinal sections of the callus showed the existence of meristematic cells, which could give rise to plant regeneration. In the process of organogenesis, adventitious shoots emerged from leaf and stem explants within 2 months after culturing on MS media fortified with 6-Benzylaminopurine (1.0-2.0 mg/l). Ultimately, in vitro germination was successfully established for the propagation and future conservation programs of this species.