Efecto in vitro del levamisol sobre las celulas mononucleares de la sangre periférica de trucha arcoíris (Oncorhynchus mykiss)

Leonardo A. Gómez; Raul A. Cortes; Carlos T. Smith

Authors

Leonardo A. Gómez Universidad de Concepción, Concepción, Chile.
Raul A. Cortes
Carlos T. Smith Universidad de Concepción, Concepción, Chile.

Keywords:

Innate immunity, reactive oxygen species, ROS, nitric oxide

Abstract

The in vitro effect of levamisole (1, 10 and 100 ?g/ml) on cells of the innate immune response of rainbow trout (Oncorhynchus mykiss) was studied, particularly the activity of natural cytotoxic cells and macrophages present in peripheral blood. Levamisole effect on natural cytotoxic cells was evaluated by their capacity to lyse HL-60 tumor cells while activity of macrophages was evaluated measuring production of reactive oxygen species and nitric oxide. Results indicate that natural cytotoxic cells activity was significantly increased by 1 ?g/ml levamisole and that higher doses had no effect. On the other hand, reactive oxygen species and nitric oxide production by macrophages was not increased by any of the levamisole doses used and that the higher dose (100 ?g/ml) induced a significant decrease of nitric oxide levels, which might be deleterious for the defensive response of these fish.

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Author Biographies

Leonardo A. Gómez, Universidad de Concepción, Concepción, Chile.

Department of Microbiology, Faculty of Biological Sciences, University of Concepción, Concepción, Chile. P. O. Box 160-C, Concepción, Chile.

Raul A. Cortes

Department of Fish Physiology and Biotechnology, Instituto de Acuicultura de Torre de la Sal (IATS), Consejo Superior de Investigaciones Científicas (CSIC), Castellon, Spain.