Description of the limbo of Piper aduncum L. (Piperaceae): Structural analysis, histochemical and essential oils

Abstract

Piper aduncum L. is a shrub specie, found mainly in soil with elevated organic matter and humidity. The essential oil containing insecticide and bactericide action, and also has broad application in agricultural and medicinal areas. The objective of this study is to characterize the lamina of P. aduncum. There was realized anatomical and histochemical characterization of paradermic, and anatomical characterization of transversal sections from apical region, median and basal of leaf blade, prepared according to usual technique of light and electronic microscopy of scaning. The mesophyll is dorsiventral with subcelular layer in both epiderms. In the epidermis and subcelular layers, it was only found the presence of total protein. In the palisade and spongyous parenchyma were found starch total and insaturated lipids. In the fundamental parenchyma there were visualized starch, protein, total lipids, ortodihidroxiphenols and terpenoid with carbonil group. There are present unicellular idioblasts in subcelular layers, epiderm or mesophyll. It is hipoestomatic, contains tetracitic stomates. There were observed short trichome tector unicellular with fine cap, pluricelular with fine cap and glandular saclike trichome and perolated glandula. In the basal portion of the tector trichomes there were found total protein. In the oil idioblast there were observed the presence of total lipids, ortodihidroxiphenols, sesquiterpenic lactones and terpenoid with carbonil group, while it was possible to observe in the sac-like trichomes, protein, total lipids and ortodihidroxiphenols.